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. 2015 Nov 25;6:180. doi: 10.3389/fendo.2015.00180

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Copyright © 2015 Kasahara, Tateishi, Hiraoka, Otsuka, Mizukami, Ozawa, Sato, Hidema and Nishimori.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Excessive lipid accumulation in the BAT of Oxtr^−/− mice was recovered by in AAV-Oxtr injection. Hematoxylin and eosin stained brown (A–D) and white (F–I) adipose tissues from Oxtr^+/+ mice (A,F), Oxtr^−/− mice (B,G), Oxtr^-/− mice with AAV-Oxtr injection into RMR (C,H) and Oxtr^−/− mice with AAV-LacZ injection into RMR (D,I). (E) Areas of lipid droplets in BAT of Oxtr^+/+ (n = 5), Oxtr^−/− (n = 7), +AAV-Oxtr (n = 6) and +AAV-LacZ (n = 6) mice. (J) Size of adipocytes in WAT of Oxtr^+/+ (n = 4), Oxtr^−/ (n = 5), +AAV-Oxtr (n = 5), and +AAV-LacZ (n = 6) mice. *; p < 0.05, ** p < 0.01, significant differences between groups. Scale bar: 60 μm.