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. 2015 Nov 25;6:180. doi: 10.3389/fendo.2015.00180

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Copyright © 2015 Kasahara, Tateishi, Hiraoka, Otsuka, Mizukami, Ozawa, Sato, Hidema and Nishimori.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression level of thermoregulation-related genes in BAT were quantified in Oxtr^+/+, Oxtr^−/−, +AAV-Oxtr, and +AAV-LacZ mice. (A) UCP1, (B) α2A-AR, and (C) β3-AR expression were measured by quantitative PCR analysis. Mice used for the preparation of mRNA were treated in 25 and 5°C conditions (n = 5 for each groups and conditions). * p < 0.05, ** p < 0.01, *** p < 0.001, compared with 25°C condition; ^†, p < 0.05, compared with Oxtr^+/+ mice; ^##, p < 0.01, significant difference between groups.