Table 2.
Biodistribution of functionalized DSPC/Chol/DSPE–PEG2000/DSPE–PEG2000–DTPA–In (64.5:30.5:3.5:1.5) liposomes injected in LS174T-xenografted nude mice (0.5 nmol of hapten/mouse and 1–5 μCi for each radionuclide: 111In and 125I) 24 h after bispecific antibody (130 μg hMN14 × 734).
| % ID/g |
111In |
125I |
||||
|---|---|---|---|---|---|---|
| Tissue | 3 h (n = 4) | 24 h (n = 4) | 48 h (n = 4) | 3 h (n = 4) | 24 h (n = 4) | 48 h (n = 4) |
| Tumor | 3.6 ± 0.2 | 7.5 ± 2.4 | 5.6 ± 1.1 | 2.5 ± 1.0 | 5.1 ± 2.5 | 1.2 ± 0.3 |
| Blood | 20.3 ± 2.5 | 2.9 ± 1.0 | 0.5 ± 0.2 | 18.0 ± 3.5 | 2.1 ± 0.6 | 0.3 ± 0.1 |
| Liver | 11.4 ± 1.1 | 16.5 ± 2.4 | 15.3 ± 2.0 | 6.7 ± 1.3 | 4.1 ± 1.0 | 0.5 ± 0.1 |
| Kidneys | 6.9 ± 1.0 | 8.8 ± 1.7 | 7.6 ± 2.4 | 3.5 ± 0.7 | 2.1 ± 0.8 | 1.1 ± 0.3 |
| Intestine | 2.8 ± 1.0 | 2.7 ± 1.5 | 3.0 ± 0.6 | 2.0 ± 0.6 | 1.4 ± 0.7 | 0.5 ± 0.2 |
| Lung | 7.0 ± 1.1 | 2.0 ± 0.7 | 1.3 ± 0.3 | 5.1 ± 1.4 | 1.4 ± 0.6 | 0.25 ± 0.08 |
| Muscle | 0.6 ± 0.1 | 0.5 ± 0.1 | 0.42 ± 0.09 | 0.35 ± 0.07 | 0.16 ± 0.05 | 0.04 ± 0.01 |
| Spleen | 21.2 ± 1.9 | 34.3 ± 3.5 | 29.4 ± 2.4 | 8.7 ± 0.3 | 9.1 ± 3.7 | 4.3 ± 2.2 |
| Skin | 1.8 ± 0.4 | 4.2 ± 1.6 | 4.1 ± 0.5 | 1.0 ± 0.3 | 1.6 ± 0.9 | 0.6 ± 0.1 |
| Brain | 1.1 ± 0.4 | 0.16 ± 0.04 | 0.08 ± 0.01 | 0.8 ± 0.3 | 0.12 ± 0.04 | 0.014 ± 0.003 |
| Heart | 4.8 ± 2.2 | 2.2 ± 0.7 | 2.0 ± 0.4 | 4.8 ± 1.8 | 1.3 ± 0.4 | 0.6 ± 0.2 |
| Bone | 3.4 ± 0.7 | 2.0 ± 0.6 | 1.6 ± 0.2 | 2.3 ± 0.5 | 0.6 ± 0.3 | 0.13 ± 0.04 |
| Stomach | 0.8 ± 0.1 | 0.8 ± 0.5 | 0.6 ± 0.4 | 0.66 ± 0.09 | 0.5 ± 0.3 | 0.18 ± 0.09 |
The data are expressed as the percentage of total administered dose per gram of tissue (% ID/g, mean ± SD).