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. 2015 Nov 25;5:16719. doi: 10.1038/srep16719

Figure 5. Increased Th2 cytokine production in splenocytes of GPRC6A−/− mice.

Figure 5

(a) Splenocytes from 8 wildtype (wt, black) and 7 GPRC6A−/− mice (white) immunized with Ova/Alum (25 days) were in vitro restimulated with Ova and supernatants were harvested 3 days later. Cytokine concentrations were measured by CBA. (b) Splenocytes from 9 wildtype (wt, black) and 9 GPRC6A−/− mice (white) immunized with Ova/Alum (25 days) were in vitro restimulated with Ova and CD4+ T cell proliferation using CFSE staining was measured. (c) Ex vivo Tfh frequencies in splenocytes from 6 wildtype (wt, black) and 7 GPRC6A−/− mice (white) immunized with Ova/Alum (25 days). (d–f) Splenic B cells from 7 wildtype (wt, black) and 7 GPRC6A−/− mice (white) immunized with Ova/Alum (6 or 8 days) were in vitro restimulated for one day with PMA/Ionomycin (e) or 5 days with Ova and anti-CD40 antibodies (d,f). Antibody titers and IL-10 concentrations were measured by ELISA. (a-f) Statistical analysis was performed using t-test. Bars represent mean ± SEM. (*P < 0.05, **P < 0.01).