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. 2015 Dec;355(3):496–505. doi: 10.1124/jpet.115.227629

Fig. 2.

Fig. 2.

Morphine withdrawal alters NMDA receptors in vivo and in vitro. (A) pNR1 was similar between the saline (N = 7)- and morphine (N = 10)-treated groups but increased about 50% during acute withdrawal (N = 9) [one-way ANOVA, F(2,23) = 3.457, P = 0.0487]. *Significant difference with a one-way ANOVA. (B) pNR1 was similar between the saline- and morphine-treated primary neuronal cultures but increased about 40% after naloxone-induced acute withdrawal [one-way ANOVA, F(2,16) = 3.892, P = 0.0420]. *Significant difference with a one-way ANOVA. (C) NR1 surface expression also increased during naloxone-induced withdrawal but not with morphine alone [one-way ANOVA, F(2,77) = 7.322, P = 0.0012]. *P < 0.05 and **P < 0.01, Bonferroni’s post hoc test. (Right side) Dual-labeled immunohistochemistry with antibodies to the NR1 subunit of the NMDAR and the MOR and fluorescent secondary antibodies colocalize in neurons harvested from E17 embryos.