Fig. 5.

Effects of MVA and SQ1 treatment on rat SULT1C2 promoter activity. A firefly luciferase reporter construct containing ∼3.1 kb of the SULT1C2 5′-flanking region (r1C2-1) and 4 deletion reporter constructs (r1C2-2 to r1C2-5) were prepared as described in Materials and Methods. Twenty-four hours after plating, primary cultured rat hepatocytes were transfected with a SULT1C2 reporter plasmid or with the empty vector as a control (pGL4.10). The next day, the hepatocytes were treated with Williams’ E medium alone (CON: control) or containing SQ1 (0.1 µM) or MVA (5 mM). The medium containing the treatments was replaced after 24 hours. Ninety-six hours after plating, cells were harvested for the measurement of luciferase activity. Bars represent mean ± S.E.M. of luciferase measurements (firefly/Renilla) normalized to untreated, empty vector controls. The results are combined from three independent experiments, with each independent experiment representing one rat hepatocyte preparation (n = 3 wells/treatment/experiment). A statistically significant treatment X plasmid interaction (P < 0.05) was detected. For clarity, only statistically significant treatment effects within individual plasmids and overall significant differences between plasmids (constructs) are shown. *Statistically significant effect of treatment (MVA or SQ1) within each reporter construct compared with the untreated, construct-matched controls (CON) (P < 0.05). Different letters denote significant differences between constructs on luciferase reporter activity (P < 0.05).