Fig. 8.

Overexpression of HNF1α and HNF1β activates the rat SULT1C2 promoter in HepG2 cells. HepG2 cells were transiently transfected with 50 ng of an HNF1α or HNF1β expression plasmid or pcDNA3.1 control plasmid together with the r1C2-2 reporter, the r1C2-2 reporter containing a mutated HNF1 site (r1C2-2mut), or with an empty vector control (pGL4.10), as described in the Materials and Methods. Forty-eight hours after transfection, cells were harvested for the measurement of luciferase activity. Bars represent the mean ± S.E.M. of luciferase measurements (firefly/Renilla) normalized to the empty vector controls. All results are combined from three independent experiments (n = 3 wells/treatment/experiment). For clarity, only significant treatment effects within individual plasmids and significant differences among plasmids (constructs) are shown. *Statistically significant effect of the HNF1 expression plasmid within each reporter construct compared with the construct-matched pcDNA3.1 control (CON) (P < 0.05). Different letters denote significant differences among the individual constructs on luciferase reporter activity (P < 0.05).