TABLE 1.

Chromatographic protocols

The aqueous solvent (A) was 0.1% acetic acid in water (v/v) and the organic solvent (B) was 0.1% acetic acid in acetonitrile (v/v). The autosampler chamber was held at 6°C, and columns were held at 25°C for all experiments. The protocol for BPA in vivo is based on that of Yokota et al. (1999).

Assay	Column	λ	Flow Rate	Elution Program
		nm	ml/min
Naproxen in vitro	Zorbax Eclipse XDB-C18 (150 × 4.6 mm, 5µm)	225	1.50	30% B from 0 to 5 minutes
				Linear increase to 70% B from 5 to 5.5 minutes
				Linear increase to70% B from 5.5 to 10 minutes
BPA in vitro	Zorbax Eclipse XDB-C18 (150 × 4.6 mm, 5µm)	280	0.50	15% B from 0 to 4.5 minutes
				Linear increase to 60% B from 4.5 to 5 minutes
				Linear increase to 60% B from 5 to 16 minutes
BPA in vivo	Tosoh TSKgel ODS 80TM (250 × 4.6 mm, 5 µm)	280	1.00	Isocratic 35% B