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. 2015 Nov 25;5:16991. doi: 10.1038/srep16991

Figure 1. Single-agent activities of 54 therapeutic compounds screened against 5 osteosarcoma cell lines.

Figure 1

(a) Percent reduction in CellTiter-Glo signal indicating decreased ATP production at 72 hours post-drug treatment (normalized to vehicle controls). (b) Relative fold change in Caspase-Glo signal indicating cell death 24 hours post-drug treatment (normalized to vehicle controls). (c) Immunocytochemistry staining of caspase-3 activity in MG63 cells after 6-hour treatment with GSK923295A (7122 ng/mL) showing caspase-3 up-regulation in response to drug treatment. (d) Top 10 drug candidates with diverse mechanisms-of-action were selected from single-agent screening results to be further evaluated in 45 drug combinations for synergistic effects against pediatric osteosarcoma. N = 3 biological replicates. Scale bar: 20 μm.