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. 2015 Nov 25;6:1041. doi: 10.3389/fpls.2015.01041

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Copyright © 2015 Jiang, Yu, Fang, Cao, Ma and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Fluorescence quenching assay showing the activation of conductance regulator (CFTR) chloride channel activity by kobusin and eudesmin in transfected FRT cells. (A) Original traces showing quenching of YFP fluorescence by I^- addition with PBS and after additions of indicated concentrations of kobusin and Gen (50 μM). (B) Original traces showing quenching of YFP fluorescence by I^- addition with PBS and after additions of indicated concentrations of eudesmin and Gen (50 μM). (C) Dose-dependent activation of CFTR chloride channel activity by kobusin and eudesmin. (D) Activation of CFTR Cl^- channel activity by kobusin and eudesmin in the presence of gradient concentrations of CFTR inhibitor CFTR_inh-172. Dose-response effects of kobusin (E) and eudesmin (F) on the activation of CFTR in the presence of different concentrations of FSK. Data are the means ± SEs of three independent tests.