Neutralizing effects of TNF-α-specific antibodies. (A) L929 cells were treated with mTNF-α and serially diluted sera, and protective rates were calculated. Data represent mean protective rates ± SD for n = 3 mice/group. ***, P < 0.001 (compared with the results from the PB control group). (B and C) Mice were treated with DSS to induce acute colitis and administered sera from hTNF-α- or mTNF-α-vaccinated mice. Mice were sacrificed on day 7 after DSS treatment, and colons were collected for histological examination. (B) Data represent mean DAI scores ± SD for n = 6 mice/group. *, P < 0.05; **, P < 0.01 (compared with the results from the PB control group). (C) Data represent mean histological scores ± SD for n = 6 mice/group. **, P < 0.01 (compared with the results from the PB control group).