Fig. 3.
Competition binding curves of ligands to turkey β1AR and human β2AR. Inhibition of 3H-CGP12177 binding to (A) CHO cells stably expressing the turkey β1AR (CHO-tβ1) and (B) CHO-hβ2 cells was measured in response to cyanopindolol (filled circles), 7-methylcyanopindolol (open circles), carazolol (filled triangles), 2-hydroxy-5-(2-[(hydroxy-3-[4-(1-methyl-4-trifluoromethyl-2-imidazolyl)phenoxy]propyl)amino]ethoxy)benzamide (open inverted triangles), and ICI118551 (stars). Log KD values are given in Table 1. Bars to the left of the graphs represent total 3H-CGP12177 binding (filled bar) and nonspecific binding (hatched bar) as determined in the presence of 10 μM of propranolol. The concentrations of 3H-CGP12177 present were (A) 0.98 nM and (B) 0.53 nM. Data points are the mean ± S.E.M. of triplicate determinations, and these single experiments are representative of (A) five and (B) 10 separate experiments.