Figure 4.

Location of melanocytes around the pterygopalatine artery. (A) RT-PCR performed on cDNA obtained from B16 mouse melanoma cells as positive control, olfactory bulb meninges from C57Bl/J6, B6Tyr^c-2J/Tyr^c-2J and Mitf^mi−vga9/Mitf^mi−vga9 mice and no-cDNA negative control. Primers used were for Mitf-M, Pmel, and Actin. (B) A coronal section of the pterygopalatine artery stained with H&E. (C) An image of an adjoining section stained with Masson-Hamperl silverstain to detect the presence of melanin. Pp, pterygopalatine artery. Scale bars represent 100 μm.