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. 2015 Nov 25;16:82. doi: 10.1186/s12868-015-0222-y

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© Hou et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Co-treatment with autophagy inhibitors attenuated neuroprotection of lithium and increased vulnerability of SH-SY5Y to rotenone. Cells were pretreated with autophagy-related drugs for 24 h followed by rotenone exposure for another 24 h. The morphology changes of cells were shown in (a).The cell viability was measured by MTT assay (b) or Annexin-V/PI staining by flow cytometry (Fig. 3a). *P < 0.05 versus control; ^# P < 0.05 versus rot alone; **P < 0.05 as compared with LiCl + Rot group; ^## P < 0.05 as compared with LiCl + Rot group (n = 6 in each group)