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. 2015 Nov 25;16:82. doi: 10.1186/s12868-015-0222-y

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© Hou et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Co-treatment with autophagy inhibitors depressed lithium induced autophagy in SH-SY5Y cells. The cells were treated with autophagy-related drugs at various concentrations for 24 h. The induction of autophagy was determined by measuring the LC3 protein levels using immunoblotting assay with antibody against LC3 (b). β-actin was used as an equal loading of proteins. The ratio of LC3B-II/I was evaluated by densitometric analysis and data were expressed (a). *P < 0.05 as compared with Con group (n = 6 in each group)