Table 1.
Sequencing metrics of the analyzed VH sequences
| Sequencing results | Parameter | Totalsa | Amplicon library (Average ± SD)b |
|---|---|---|---|
| Raw output | 454 reads | 1,792,504 | 40,738 ± 27,496 |
| Total bases (bp) | 778,487,667 | 17,692,901 ± 12,073,169 | |
| Average length (bp) | ND | 483 ± 77 | |
| Quality and size filters | Eliminated reads (%) | 27 % | 24 % ± 14 |
| Sequences included in the analysis | Analyzed with ImmunediveRsity | 1,302,865 | 29,610 ± 18,114 |
| Clonotypes | 71,568 | 1,626 ± 938 | |
| Lineages | 218,910 | 4,975 ± 3,378 |
aTotal parameters of five 454-Roche Titanium sequencing runs. Forty-four gene libraries were generated from 13 individuals
bEach gene library corresponds to one of six conditions: day 0 and day 7 after vaccination with TIV2008/2009, day 0 and day 7 after vaccination with 09 MIV2009, and day 0 and day 7 after vaccination with TIV2014/2015