Figure 4.

Analysis of apoptosis of aortic endothelial cells by Hochest33342/PI staining. The isolated cells were divided into negative control group (aorta endothelial cells isolated from SD rats), spontaneous hypertension group (aorta endothelial cells isolated from rats with spontaneous hypertension), berberine group (aorta endothelial cells isolated from rats with spontaneous hypertension + berberine). Cells in berberine group were treated with berberine (1.25, 2.5, and 5 μmol/L) for 24 h. Cell apoptosis was analyzed by Hochest33342/PI staining.