Figure 5.

Effects of HMGB-incubated SIRT1-/-human OS cells on VEGF expression. Upper part of this figure demonstrated the immunoblots of VEGF and SIRT1 in HMGB-incubated SIRT1/human OS cells. Ctrl: human OS cells; siRNA: human OS cells incubated with SIRT1 siRNA; Ctrl+Emodin: human OS cells incubated with emodin; siRNA+Emodin: SIRT1/human OS cells incubated with emodin. ^ADifferences were significantly from Ctrl, ^Bdifferences were significantly from siRNA; ^Cdifferences were significantly from Ctrl+Emodin. ^aDifferences were significantly from Ctrl; ^bdifferences were significantly from HMGB.