Skip to main content
. 2015 Sep 15;8(9):15087–15097.

Figure 2.

Figure 2

Growth curves of and TLR-4 expression in the osteoblast MG-63 cells treated with HMGB1 under normoxia or hypoxia. (A and B) Growth curve of HMGB1-treated osteoblast MG-63 cells under normoxia (A) or under hypoxia (B) MG-63 cells with more than 85% confluence were treated with 0, 0.2 or 1 μg/mL HMGB1 for 24, 48 or 72 hours, under normoxia or under hypoxia, and then were examined with CCK-8 assay. (C) Western blot analysis of Toll-like receptor (TLR)-4 in U937 cells which were treated with or without 0.2 μg/mL HMGB1, under normoxia or under hypoxia for 24 or 48 hours, with tubulin as internal control; (D) Relative level of TLR-4 to tubulin in U937 cells. Results were repeated in triplicate and were shown as Mean ± SE. * or #P < 0.05, ** or ##P < 0.01.