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. 2015 Sep 15;8(9):15087–15097.

Figure 4.

Figure 4

Growth curves of HMGB1-treated MG-63 cells post the TLR-4 knockout under normoxia or hypoxia. (A) The relative level of Toll-like receptor (TLR)-4 in MG-63 cells which were transfected with 30 or 60 NM TLR-4-specific SIRNA (SIRNA-TLR-4) or with control SIRNA (SIRNA-Con) for 12 hours; (B) Western blotting of TLR-4 in the siRNA-TLR-4- or siRNA-Con-transfected MG-63 cells under hypoxia for 24 hours; (C and D) Growth curve of siRNA-TLR-4- or SIRNA-Con-transfected MG-63 under normoxia (C) or under hypoxia (D). MG-63 cells with more than 85% confluence were transfected with 30 or 60 NM SIRNA-TLR-4 or with siRNA-Con were incubated under normoxia or under hypoxia for 24, 48 or 72 hours, and then were examined with CCK-8 assay. All experiments were performed respectively in triplicate. *P < 0.05, **P < 0.01.