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. 2015 Sep 15;8(9):15108–15116.

Figure 1.

Figure 1

Let-7 was downregulated in HepG2 cells that were stably and transiently transfected with HBx plasmid. A. Let-7 expression was measured using qRT-PCR and normalized by U6 expression in HepG2 cells that stably expressed HBx or control vector. B. The expression of let-7 normalized to U6 was detected by qRT-PCR in HepG2 cells transiently transfected with HBx-expressing plasmid or control vehicle. HepG2 cells were transfected with 0.6, 0.8 and 1 µg PCDNA3.1-hbx or 0.6 µg PCDNA3.1 as a control. Cells were collected for analysis 48 h after each transfection. Data are presented as the mean ± SE fold change. (*P < 0.05, **P < 0.01, ***P < 0.001; Student’s t-test; n=3).