Figure 2.

Lin28B was overexpressed in HepG2 cells expressing HBx and HBV⁺ HCC tissues. (A) qRT-PCR and (B) representative western blot results demonstrated that HBx upregulated Lin28B transcription and translation in HepG2 cells. Relative Lin28B expression was normalized to GAPDH. The qRT-PCR data are presented as the mean ± SE fold change compared with HepG2-vc (*P < 0.05, **P < 0.01, ***P < 0.001; n=3, Student’s t-test). (C) The expression of Lin28B protein was detected by IHC. Representative results are presented.