Figure 4.

Effect of Lck siRNA on markers of inflammation. Mice were sensitized with OVA and transfected via tail vein injection with nonspecific siRNA or Lck siRNA, and then challenged with OVA. Control mice were sensitized and challenged with PBS and untransfected. Following OVA challenge, mice were sacrificed and BALF and plasma were collected. The total number of leukocytes (A) and the percentage of eosinophils (B) in BALF were determined. BALF was analyzed by ELISA to determine the concentration of IL-4 (C) and IgE (D). Plasma was analyzed by ELISA to determine the concentration of IL-4 (E) and IgE (F). *P < 0.05, **P < 0.01, compared to nonspecific siRNA (n=9 per group).