Figure 4.

Vitamin C inhibits HIF-1α-induced proliferation and migration of lens epithelial cells. HLE-B3 cells were stably transfected with wild-type HIF-1α or proline-mutant HIF-1α or negative control. Cells expressing wild-type HIF-1α or proline-mutant HIF-1α were treated with PBS vehicle control or vitamin C (100 μM) during the experiments. A. Representative photographs of colony formation in HLE-B3 cells are shown on the left. Quantification of the colony formation rate is shown on the right; *P<0.003 compared with the control group. B. The migration potentials of HLE-B3 cells with different treatments were determined by transwell assay. The representative images are shown on the left, and the quantification of selected fields is shown on the right; *P=0.002 compared with the control group.