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. 2015 Sep 15;8(9):15190–15197.

Figure 4.

Figure 4

The combined administration of LUT and DOX enhances autophagy and apoptosis in human osteosarcoma U2OS cells. A. U2OS cells were grown on glass slides in 24-well plates at 50% confluent, and then treated with 100 µM LUT, 100 µM DOX, 100 µM DOX plus 100 µM LUT for 24 h (PBS as a bank control). After treatment, cells were stained with AO and observed with fluorescence microscopy to detect the presence of AO puncta. B. Electron micrographs of U2OS cells after treatment for 24 h with 100 µM LUT, 100 µM DOX, 100 µM DOX plus 100 µM LUT for 24 h (PBS as a bank control). C. Annexin V-FITC and PI staining for apoptosis. X-axis, Annexin V-FITC; Y-axis, DNA content by propidium iodide. The test was repeated three times and the image presented was typical of these three independent tests.