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. 2015 Sep 15;8(9):15413–15422.

Figure 2.

Figure 2

Affections of NAC on emodin-induced ROS production and apoptosis in SW480 and SW620 cells. A. Demonstrated the fluorescent images of Hoechst and DCFH-DA staining. Hoechst staining is used to mark the apoptotic cells while DCFH-DA staining is considered a reliable method to detect intracellular ROS production. The upper part demonstrated Hoechst and DCFH-DA staining of SW480 and SW620 cells incubated with serially diluted emodin solutions; the lower part showed the Hoechst and DCFH-DA staining of SW480 and SW620 cells received NAC and/or emodin treatments. B. Columns demonstrated the apoptotic percentage of SW480 and SW620 cells incubated with serially diluted emodin solutions. [*differences were significant when compared with previous concentration]. C. Columns demonstrated the mean fluorescent intensity of DCFH-DA staining in SW480 and SW620 cells incubated with serially diluted emodin solutions. [*differences were significant when compared with previous concentration]. D. Columns demonstrated the apoptotic percentage of SW480 and SW620 cells received NAC and/or emodin treatments. [a. differences were significant when compared with “Ctrl”; b. differences were significant when compared with “Ctrl+NAC”; c. differences were significant when compared with “emodin”]. E. Columns showed the mean fluorescent intensity of DCFH-DA staining in SW480 and SW620 cells received NAC and/or emodin treatments. [a. differences were significant when compared with “Ctrl”; b. differences were significant when compared with “Ctrl+NAC”; c. differences were significant when compared with “emodin”].