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. 2015 Sep 15;8(9):15413–15422.

Figure 4.

Figure 4

Effects of Puma silencing on emodin-induced ROS production, apoptosis and p38/p53/Puma signaling. A. Demonstrated the captured fluorescent images of Hoechst and DCFH-DA staining on SW480 and SW620 cells received Puma siRNA and/or emodin treatments. B. Demonstrated the immunoblots of phosphorylated p38, total p38, p53, histone, Puma and GAPDH in SW480 cells received Puma siRNA and/or emodin treatments. C. Columns demonstrated the apoptotic rate of SW480 and SW620 cells received Puma siRNA and/or emodin treatments; D. Columns demonstrated the mean fluorescent intensity of DCFH-DA staining of SW480 and SW620 cells received Puma siRNA and/or emodin treatments; E. Columns demonstrated the p-38 phosphorylation level (p-p38/p38), p53 unclear translocation (p53/histone) and Puma expression (Puma/GAPDH) in SW480 cells received Puma siRNA and/or emodin treatments. [a. differences were significant when compared with “Ctrl”; b. differences were significant when compared with “Ctrl+Puma-/-”; c. differences were significant when compared with “emodin”]. The results immunoblots of phosphorylated p38, total p38, p53, histone, Puma and GAPDH in SW620 cells were not shown in this figure which were similar to results in SW480 cells.