Figure 4.

ING5 inhibits PI3K/Akt signaling pathway in breast cancer cells. A: The levels of phosphorylated PI3K, total PI3K, phosphorylated Akt and total Akt in MAD-MB-231 cells transfected with overexpression-ING5 or mock were determined by western blot. B and C: The relative protein expression levels of p-PI3K and p-Akt were quantified using Image-Pro Plus 6.0 software and normalized to β-actin. All experiments were repeated at least three times. Data are presented as mean ± SD. *P < 0.05 compared to the mock group.