Table 1.

Primers of CTLA4, PD-1 and PD-L1 SNPs used for RFLP analysis

Marker name	SNP	rs number	Location PCR primers
CTLA-4	+49A>G	rs231775	F: 5’-GCTCTACTTCCTGAAGACCT-3’
			R: 5’-AGTCTCACTCACCTTTGCAG-3’
CTLA-4	-318C>T	rs5742909	F: 5’-AAATGAATTGGACTGGATGG-3’
			R: 5’-TTACGAGAAAGGAAGCCGTG-3’
PD-1.1	-538G>A	rs36084323	F: 5’-ATCTGGAACTGTGGCCATGGTG-3’
			R: 5’-ATTCTGTCGGAGCCTCTGGGAG-3’
PD-1.3	7146G>A	rs11568821	F: 5’-CCCCAGGCAGCAACCTCAAT-3’
			R: 5’-GACCGCAGGCAGGCACATAT-3’
PD-1.5	7785T>C	rs2227981	F: 5’-GTGCCTGTGTTCTCTGTGGA -3’
			R: 5’-CCAAGAGCAGTGTCCATCCT-3’
PD-1.9	7625C>T	rs2227982	F: 5’-GGACAGCTCAGGGTAAGCAG-3’
			R: 5’-AGGGTCTGCAGAACACTGGT-3’
PD-L1	8293A>C	rs2890658	F: 5’-AATGGCTTGTTGTCCAGAGATG-3’
			R: 5’-GTACCACATGGAGTGGCTGC-3’