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. 2015 Nov 25;16:71. doi: 10.1186/s12865-015-0132-x

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© Kim et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — a Schematic diagram for construction of recombinant sRANKL expression vector system (modified from [3]). b Western blot for detecting sRANKL from cell extracts (intracellular) and concentrated culture supernatants (extracellular). C: commercial sRANKL; lane 1: sRANKL-LAB; lane 2: WT-LAB