Table 1.

Activities of iLDHs in crude cell extracts of P. putida KT2440 and P. putida. KT2440 (∆lldR) cultured with different growth substrates

Growth substrate	Strain	Enzyme activity (nmol/min mg protein)a
		l-iLDH	d-iLDH
dl-Lactate	P. putida KT2440	161.4 ± 4.6	332.9 ± 4.3
	P. putida KT2440 (∆lldR)	220.9 ± 7.0	471.5 ± 3.5
Glycerol	P. putida KT2440	ND	ND
	P. putida KT2440 (∆lldR)	348.2 ± 11.2	771.0 ± 9.0

ND not detected

^aActivities of d-iLDH and l-iLDH were examined with 20 mM d-lactate or 20 mM l-lactate. DCIP was used as the electron acceptor. Results are mean ± SD of three parallel replicates