Table 1.
IL2 and male B-type ciliated neurons release specific GFP-labeled ECV cargo
Reporter | Expression pattern and identity | Animals releasing ECVs (%) | n |
---|---|---|---|
PKD-2::GFP | Male B-type neurons, TRP polycystin-2 | 100 | 50 |
LOV-1::GFP1 | Male B-type neurons, polycystin-1 | 5.6 | 53 |
CWP-1::GFP | IL2 + male B-type neurons, secreted protein | 100 | 50 |
Posm-6::LMP-1::GFP | Pan-ciliary, LAMP1 | 13.1 | 38 |
CHE-11::GFP | Pan-ciliary IFT complex A | 0 | 20 |
OSM-6::GFP | Pan-ciliary IFT complex B | 0 | 19 |
OSM-3::GFP | Pan-ciliary kinesin-2 motor | 0 | 20 |
KAP-1::GFP | Pan-ciliary kinesin-II associated | 0 | 20 |
KLP-6::GFP | IL2 + male B-type neurons, kinesin-3 | 0 | 31 |
TBB-4::GFP | Pan-ciliary axonemal tubulin | 0 | 20 |
ODR-10::GFP | AWA, GPCR | 0 | 50 |
Ppkd-2::GFP | PKD, Soluble GFP | 0 | 20 |
All reporters use their own promoter except where noted. Transgenic animals express LOV-1::GFP1 (a fusion between the extracellular domain of LOV-1 (1–991 amino acids and GFP) at low levels and in a mosaic pattern, which is reflected in the lower number of animals releasing ECVs. Adult males and hermaphrodites were examined.