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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Curr HIV Res. 2016;14(1):47–53. doi: 10.2174/1570162x1401151102125319

Figure 2. Immunohistochemical analysis of expression of TNF-α and TNFR1 in HIV-1/PML brain tissues.

Figure 2

Double immunofluorescence labeling of HIV-1/PML with (A, B) anti-TNF-α (red) and anti-GFAP (green), (C, D) anti-TNFR1 (red) and anti-GFAP (green). (A–D) Arrowheads indicate astrocyte immunoreactivity with TNR-α or TNFR1, arrows indicate GFAP negative, TNFR1 and TNF-α positive cells. (E, F) Hematoxylin and Eosin (H&E) stain, arrowheads indicate nuclear inclusions in bizarre astrocytes. (A, C, E) 400× magnification, (B, D, F) 400× magnification enlarged to show detail.