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Current traces induced by interactions of PNP with DOPC BLM. Symmetric solutions of 10 mM KCl bathed both sides of BLM and a transmembrane potential of +100 mV was applied. Currents were induced by addition of 100 μg/ml of COOH-PNP (A) or 50 μg/ml amidine-PNP (B, C) to the cis side. Transition of current induced by amidine-PNP from low to high amplitude state is shown in C. Dotted line indicates zero current.
