Figure 2.

Western blotting and quantification of the APP-α-processing after RNAi or overexpression of ACAT1 (A) The human SK-N-SH cells were transiently transfected with duplexes of siRNA targeting ACAT1 mRNA. After a 48-h transfection, the immunoblotting of the cultured media and cell lysates as well as the quantification of the sAPPα, APP, or ACAT1 protein were performed according to the procedures described in the ‘Materials and Methods’. The data were the mean ± SD from three quantifications. The relative levels of proteins were expressed as fold to the controls without transfection. (B) The human SK-N-SH cells were transiently transfected with the expression plasmid pACAT1. After a 24-h transfection, the data were obtained by the same methods as indicated in (A).