Figure 5.

Determinations of the PM-FC and the APP-α-processing after culture under different conditions (A) The human SK-N-SH cells were incubated in normal media with 10% FBS or cholesterol-depleting media with 5% LPDS plus Lova (1 µM) for 12 h, followed by the incubation in fresh individual media with CP at different concentrations for another 12 h. The PM-FC and TC of the treated cells were determined according to the procedures described in the ‘Materials and Methods’. The data were the mean ± SD from three independent experiments. The relative PM-FC were expressed as fold to the control cultured in FBS without the CP treatment. (B) The human SK-N-SH cells were cultured and treated as indicated in (A). The immunoblotting of the cultured media and cell lysates was performed according to the procedures described in the ‘Materials and Methods’. (C,D) The human SK-N-SH cells were incubated in cholesterol-depleting media with 5% LPDS plus Lova (1 µM) for 12 h, followed by changing to the fresh media with or without 16 µM CP under different concentrations of the LDL (2 and 10 μg/ml) or Mev (0.5 and 2.5 mM) for another 12 h. The data were obtained by the same methods as indicated in (A) and (B), respectively. The relative PM-FC were expressed as fold to the control without the CP, LDL, and Mev treatment. ***P < 0.001; **P < 0.01; *P < 0.05.