TABLE 1 .
Genes required for growth of A. baumannii in G. mellonellaa
| Function and annotation | Gene | Product | RR |
|---|---|---|---|
| Micronutrient acquisition | |||
| ABUW_1173 | bauD | Acinetobactin permease | 0.0119 |
| ABUW_1174 | bauC | Acinetobactin permease | 0.0976 |
| ABUW_1176 | bauB | Acinetobactin periplasmic binding protein | 0.0472 |
| ABUW_1177 | bauA | Acinetobactin receptor | 0.0111 |
| ABUW_3740 | znuA | High-affinity Zn transport protein | 0.0312 |
| ABUW_3741 | Zur | Fur family transcriptional regulator | 0.0730 |
| ABUW_3742 | znuC | Zinc import ATP-binding protein | 0.0685 |
| ABUW_3743 | znuB | High-affinity Zn transport protein | 0.0449 |
| Cysteine metabolism/sulfur assimilation | |||
| ABUW_0643 | cysI | Sulfite reductase | 0.0217 |
| ABUW_0722 | cysH | PAPSb reductase | 0 |
| ABUW_0853 | cobA | Uroporphyrin-III C-methyltransferase | 0 |
| ABUW_1760 | Sulfate permease | 0.0959 | |
| ABUW_2218 | cysQ | 3′(2′),5′-Bisphosphate nucleotidase | 0.0503 |
| ABUW_2362 | cysE | Serine acetyltransferase | 0.0361 |
| ABUW_2895 | cysN | Sulfate adenylyltransferase subunit 1 | 0 |
| ABUW_2896 | cysD | Sulfate adenylyltransferase subunit 2 | 0 |
| Aromatic hydrocarbon metabolism | |||
| ABUW_1835 | pcaD | 3-Oxoadipate enol-lactonase | 0.0469 |
| ABUW_1837 | pcaC | 4-Carboxymuconolactone decarboxylase | 0.0652 |
| ABUW_1848 | pcaU | pca operon regulatory protein | 0.0852 |
| ABUW_1854 | benP | Benzoate transport porin | 0.0305 |
| ABUW_2090 | 4-Hydroxybenzoate transporter | 0.0909 | |
| ABUW_2349 | 4-Oxalocrotonate tautomerase | 0.0086 | |
| ABUW_2374 | Isochorismatase hydrolase | 0.0631 | |
| ABUW_2523 | paaI1 | Thioesterase domain protein | 0.0069 |
| ABUW_2524 | paaY | Phenylacetic acid degradation protein | 0.0934 |
| Cell envelope/membrane/Wall | |||
| ABUW_3360 | lptE | LPS assembly | 0 |
| ABUW_3447 | lpxL | Lipid A biosynthesis acyltransferase | 0 |
| ABUW_3448 | lptB | Glycosyl transferase, group 1 | 0.0248 |
| ABUW_3638 | pbpG | d-Alanyl-d-alanyl carboxypeptidase | 0 |
| ABUW_3831 | Wza | Polysaccharide export protein | 0.0133 |
| ABUW_3832 | Ptp | Protein tyrosine phosphatase | 0 |
| ABUW_3833 | Ptk | Tyrosine protein kinase | 0 |
| Stress response genes | |||
| ABUW_0655 | typA | GTP-binding protein TypA/BipA | 0.0223 |
| ABUW_1595 | Kef | Ion transport protein | 0.0666 |
| ABUW_1648 | mscS | Mechanosensitive ion channel | 0.0658 |
| ABUW_1740 | rseP | Intramembrane metallopeptidase | 0.0279 |
| ABUW_1763 | uspA | UspA domain protein | 0 |
| ABUW_1804 | trkH | K+ uptake system component | 0.0639 |
| ABUW_2521 | uvrD | UvrD/REP helicase | 0.0460 |
| ABUW_2590 | kefF | NADPH oxidoreductase | 0.0405 |
| ABUW_3122 | otsB | Trehalose phosphatase | 0.0859 |
| Antibiotic resistance | |||
| ABUW_0842 | adeK | Multidrug efflux protein | 0 |
| ABUW_0843 | adeJ | Multidrug efflux protein | 0.0157 |
| ABUW_0844 | adeI | Multidrug efflux protein | 0.0154 |
| ABUW_1156 | Drug/metabolite exporter | 0.0442 | |
| ABUW_1499 | EamA-like transporter | 0.0619 | |
| ABUW_1520 | EamA-like transporter | 0.0635 | |
| ABUW_1673 | Bacterial TMc pair family protein | 0.0458 | |
| ABUW_1851 | Aminoglycoside phosphotransferase | 0.0912 | |
| ABUW_2123 | Metallo-β-lactamase family protein | 0.0828 | |
| ABUW_2550 | EamA-like transporter | 0.0584 | |
| Transcriptional regulation | |||
| ABUW_1645 | TetR family transcriptional regulator | 0.0437 | |
| ABUW_1672 | LysR family transcriptional regulator | 0.0676 | |
| ABUW_1692 | TetR family transcriptional regulator | 0.0493 | |
| ABUW_1755 | gigD | AsnC family transcriptional regulator | 0 |
| ABUW_1768 | MarR family transcriptional regulator | 0.0272 | |
| ABUW_1849 | LysR family transcriptional regulator | 0.0547 | |
| ABUW_1966 | LysR family transcriptional regulator | 0.0556 | |
| ABUW_2074 | Fur family transcriptional regulator | 0.0495 | |
| ABUW_2196 | alkR | AraC family transcriptional regulator | 0.0516 |
| ABUW_2236 | AraC family transcriptional regulator | 0.0550 | |
| ABUW_2370 | arsR | ArsR family transcriptional regulator | 0 |
| ABUW_2520 | TetR family transcriptional regulator | 0.0666 | |
| ABUW_2544 | AraC family transcriptional regulator | 0.0373 | |
| ABUW_2555 | soxR | Redox-sensitive transcriptional activator | 0.0934 |
| ABUW_3161 | gigC | LysR family transcriptional regulator | 0.0007 |
| ABUW_3180 | bfmS | TCS sensor kinase protein | 0 |
| ABUW_3260 | gigA | Putative TCS response regulator | 0.0671 |
a
Listed is a subset of genes identified in the TnSeq screen as outlined in the text. For the full list of hits, see the supplemental material.
b
PAPS, phosphoadenosine phosphosulfate.
c
TM, transmembrane.