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. 2015 Nov 25;10(11):e0143447. doi: 10.1371/journal.pone.0143447

Fig 1. SA-binding activity of HsGAPDH monitored by photoaffinity labeling with 4AzSA.

Fig 1

Crosslinking of 4AzSA to HsGAPDH is suppressed by SA. HsGAPDH was incubated without (-) or with (+) 4AzSA (0.05 mM) in the absence or presence of the indicated concentrations of SA for 1 h, and then exposed to UV light (50 mJ). Proteins crosslinked with 4AzSA were detected by immunoblot analysis using α-SA antibody. Proteins stained with Coomassie brilliant blue (CBB) served as a loading control. The experiment was repeated three times with similar results. Results are expressed as a percentage of inhibition (in lower panel) in the presence of the indicated concentrations of SA as compared to the signal observed in the absence of SA, which was assigned 0% inhibition.