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. 2015 Nov 25;10(11):e0143898. doi: 10.1371/journal.pone.0143898

Fig 2. Graphic illustration of the various regions deleted in the complementing plasmids.

Fig 2

The basic region of Hsa was located on amino acid position 228–449. pAS8741 carries an intact hsa gene; pAS8748 encodes a Hsa protein lacking the SR1 and NR2 regions (HsaΔ105–446); pAS8744 encodes a Hsa protein lacking the SR1 region (HsaΔ105–245); pAS8746 encodes a Hsa protein lacking the NR2 region (HsaΔ246–446); pAS8749 encodes a Hsa protein lacking the N-terminal part of the SR2 region (HsaΔ448–1459); pAS8375 encodes a Hsa protein lacking the C-terminal part of the SR2 region (HsaΔ1092–2143); pAS8165 encodes a Hsa protein lacking the entire SR2 region (HsaΔ448–2143); and pAS8164 encodes a Hsa protein lacking both SR1 and SR2 regions (HsaΔ105–245, Δ448–2143). The dotted lines indicate the regions deleted. BR: basic region; NR: non-repeat region; SR: serine-rich repeat region; CWAD: cell wall anchoring domain.