Table 1. Specification of immunoreagents.
| Antigen | Host species | Code | Dilution | Manufacturer/Supplier |
|---|---|---|---|---|
| Primary antibodies | ||||
| TH | Mouse | MAB 318 | 1:200 | Millipore, USA |
| DβH | Rabbit | AB1585 | 1:500 | Millipore, USA |
| NPY | Rabbit | NA1115 | 1:1000 | Biomol, Germany |
| GAL | Rabbit | AB2233 | 1:2000 | Millipore, USA |
| nNOS | Rabbit | AB5380 | 1:2500 | Millipore, USA |
| LENK | Rabbit | 4140–0355 | 1:500 | AbD Serotec, UK |
| CART | Rabbit | H-003-61 | 1:15000 | Phoenix Pharmaceuticals, USA |
| CGRP | Rabbit | AB5920 | 1:1500 | Millipore, USA |
| SP | Rat | 450–0505 | 1:200 | AbD Serotec, UK |
| VIP | Rabbit | PEPA41T | 1:1500 | AbD Serotec, UK |
| Secondary antibodies | ||||
| Alexa Fluor 488 nm donkey anti- mouse IgG | A21202 | 1:1000 | Invitrogen, USA | |
| Alexa Fluor 546 nm goat anti- rabbit IgG | A11010 | 1:1000 | Invitrogen, USA | |
| Alexa Fluor 546 nm goat anti- rat IgG | A11081 | 1:1000 | Invitrogen, USA | |