Fig 3. Characterization of the Leukemic Mice.

(A) Phenotype of the leukemic cells of the host mice that have MYC as an only proto-oncogene insert transferred by retroviral cDNA library transduction. The phenotype of the leukemic cells obtained from Mouse #3 was shown as a representative result. The leukemic cells from Mouse #7 and Mouse #9 also have MYC cDNA insert only, and the results were shown in S1 Fig. The spleen cells from Mouse #3 were stained for MAC-1, Gr-1, and B220. The genomic DNA prepared from the spleen cells was used as a template to amplify cDNA insert(s), and the PCR product(s) was separated in agarose gel. The PCR product DNA bands were excised from the gel and the identity was determined by DNA sequencing. BM: bone marrow, SP: spleen, 1^st: cells obtained from the 1^st host mice, 2^nd: cells obtained from the secondary host mice after serial bone marrow transfer. (B) A representative phenotype of the mice harboring MYC and PIM2 proto-oncogene in their leukemic cells (Mouse #5). The phenotype of the leukemic cells from Mouse #4 and Mouse #8 are shown in S2 Fig 3^rd, 4^th, 8^th: Cells obtained from the host mice after 3^rd, 4^th, and 8^th serial transfer. LN: Lymph Node. (C) A representative phenotype of the mice harboring MYC and one additional cDNA insert of which the role in leukemogenesis is uncertain. The result from Mouse #10 harboring KRAS and MYC cDNA inserts is shown. The phenotypes of the additional three mice harboring PDGFB, RAB7B, or CRX in addition to MYC are shown in S3 Fig.