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. 2015 Nov 25;10(11):e0143954. doi: 10.1371/journal.pone.0143954

Fig 4. Regulation of the RAAS and salt excretion in inducible MR knock-down mice.

Fig 4

Control (con) and transgenic (tg) mice of line B and D received Dox via food pellets for 14 days or were left untreated (w/o). Blood and urine samples were collected in the morning. (A) Serum was prepared and aldosterone levels were determined by RIA. N = 7/8 (Dox, line B), N = 7/7 (w/o, line B), N = 10/8 (Dox, line D), N = 6/8 (w/o, line D). (B) Plasma was prepared and renin levels were determined by ELISA. N = 12/8 (Dox, line B), N = 7/7 (w/o, line B), N = 9/7 (Dox, line D), N = 6/7 (w/o, line D). (C) Sodium (Na+) and potassium (K+) concentrations in the urine of mice of line B were measured using ion selective electrodes. N = 5/6. Values are depicted as mean ± SEM, statistical analysis was performed by unpaired two-tailed Student’s t test. n.s.: non-significant; *: p <0.05; ***: p <0.001.