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. 2015 Nov 25;10(11):e0143852. doi: 10.1371/journal.pone.0143852

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© 2015 Tsai et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 3 — (A) Representative micrographs of real-time confocal immunofluorescence microscopy showing RA at 0 min, 4.5 min, and 13 min. Bar scale: 30μm. (B) The distribution of Gβ (green) and RARβ (red) before RA stimulation (upper); while co-localization of them at the extended axonal membrane after RA (white arrow) (lower) and (C) a similar expression was observed in between Gα_q/11 (green) and RXRα (red). Bar scale: 30μm. (D) Representative electron micrographs showing: the subcellular large gold-tagged Gα_q/11 (20 μm, green arrow) and ER areas (blank arrow) before RA stimulation (upper). Co-localization of the small gold-tagged RXRα (6 μm, red arrow) and the large gold-tagged Gα_q/11 (green arrow) at the ER areas (blank arrow) (middle) and at the subcellular regions after RA stimulation (15 min; lower). N: nucleus, Bar scale: 1 μm.