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. 2015 Nov 25;10(11):e0143852. doi: 10.1371/journal.pone.0143852

Fig 5. Multifaceted GSK3β in the Early Morphogenesis.

Fig 5

(A-B) Dynamic expression of GSK3β by phosphorylation at Ser21/9 or Tyr279/216 sites (A) and of β-catenin and N-cadherin (B) after RA induction by immunoblotting assay. Data represent mean ± SD, n = 3, ★: p < 0.05. (C) Immunofluorescence imaging of RA induction (4 hr) showing: co-expressions of α-tubulin and MAPT at the axonal areas (arrow; upper); Parkin and α-tubulin at the subcellular regions (arrow; lower). (D-E) IP assays showing an interaction of MAPT to GSK3β-Tyr279/216 and α-tubulin (E) and to α-Synuclein (F). IgG as negative control, C as control.