Extended Data Figure 7. Comparison of GlyR with other Cys-loop receptors.

a, The two restriction sites, viewed from the cytoplasmic side. The C_α of −2′Pro equivalents (cyan) and 9′Leu equivalents (magenta) are shown as spheres. Distances between adjacent C_α atoms are labeled. b, Plot of the vector connecting the −2′ProC_α equivalent and 9′LeuC_α equivalent, with −2ProC_α equivalent as the origin, the tilt angle θ and the rotation angle φ relative to the pore axis. The φ of str-bound GlyR is arbitrarily set to zero. c, Pore radii as a function of distance along the pore axis, calculated using the program HOLE, where the C_α position of 0′Arg is set to zero. d, Table showing parameters of the vector connecting the −2′ProC_α equivalent and 9′LeuC_α equivalent, where r is the distance from −2′ProC_α equivalent to 9′LeuC_α equivalent, Restr_P and Restr_L are the pore radii at −2′Pro equivalent and 9′Leu equivalent, respectively. The d_{C loop} is the distance between C_α of Thr220 equivalent and Leu143 equivalent, representing the opening of the C loop shown in panel (e). e, Comparison of ligand binding pockets. The side chains of marker residues are shown in sticks.