Extended Data Table 1.
Statistics of 3D reconstruction and model refinement.
| strychnine | glycine | glycine/ivermectin | |
|---|---|---|---|
| Data collection/processing | |||
| Microscope | Krios | Polara | Krios |
| Voltage (kV) | 300 | 300 | 300 |
| Defocus range (μM) | −1.5–−3.0 | −1.5–−2.5 | −1.5–−3.0 |
| Exposure time (s) | 5 | 6 | 5 |
| Dose rate (e−/pixel/s) | 10.0 | 10.9 | 10.0 |
| Pixel size (Å) | 1.0100 | 1.2156 | 1.0400 |
| Particles processed | 82913 | 127276 | 160585 |
| Particles refined | 37094 | 58188 | 56957 |
| Resolution (unmasked, Å) | 4.4 | 4.4 | 4.3 |
| Resolution (masked, Å) | 3.9 | 3.9 | 3.8 |
| Map sharpening B-factor (Å2) | −146 | −151 | −156 |
|
| |||
| Refinement | |||
| Cell dimensions | |||
| a = b = c (Å) | 282.8 | 291.7 | 291.2 |
| α = β = γ (°) | 90 | 90 | 90 |
| Resolution (Å) | 3.9 | 3.9 | 3.8 |
| Number of atoms | 12705 | 12810 | 13870 |
| Protein | 12440 | 12670 | 13420 |
| Ligand | 265 | 140 | 450 |
| r.m.s. deviations | |||
| Bond length (Å) | 0.004 | 0.005 | 0.005 |
| Bond angle (°) | 0.960 | 1.115 | 1.256 |
| Ramachandran plot | |||
| Favored (%) | 97.4 | 99.1 | 99.4 |
| Allowed (%) | 2.6 | 0.9 | 0.6 |
| Disallowed (%) | 0 | 0 | 0 |