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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Immunol Cell Biol. 2015 Jun 1;93(10):896–908. doi: 10.1038/icb.2015.59

Figure 6. Primary CD4+ T cells are adequately stimulated prior to transductions & untransduced/Transduced T cells show similar levels of T cell activation markers CD44/CD69.

Figure 6

(A) Left: Un-activated and 6 day activated CD4+ T cells were surface stained with CD4+ and CD45RO.1 and analyzed using flow cytometry. Right: Quantification of surface CD45RO in primary human CD4+ T cells pre and post activation from two independent donors. (B) Untransduced and transduced primary CD4+ T cells were stained with surface CD44 or CD69 conjugated with Alexa-fluor 647 and then gated on the Large “L” population. Data was graphed as mean MFI ±SEM from three independent donors. (C) Median forward “FSC” and side “SSC” scatters were quantified from untransduced and transduced primary CD4+ T cells. Data was graphed as mean Median FSC/SSC ±SEM from three independent donors.