Figure 3.

Axon function in WT MONs is improved after IPC. Seventy-two hours after IPC pulse, axon function in MONs from WT mice was assessed. MONs were allowed to equilibrate for 30–60 min to establish a baseline (see time point a). Axons were stimulated every 30 s with a supramaximal pulse; CAPs were quantified. Error bars are shown for every 10 data points. Ischemia was induced by OGD. After 45 min of OGD, normoxic/normoglycemic control conditions were returned for a period of several hours to monitor recovery (see time point b). Insets demonstrate a typical CAP at baseline (a) and during recovery (b) in both ipsilateral preconditioned (red) and contralateral control (blue) MONs. Calibration: insets in A, 1 mV, 0.5 ms. Bar graph showing extent of CAP recovery at 60 min after the end of 45 min OGD pulse (time point b). **p < 0.01. n = 11 MONs per group.