Table 1.

Primer/probe sequences for quantification of steady-state levels of GluR 1–4 flip and flop isoforms

Gene	Isoform	GenBank accession number	Forward primer	Reverse primer	Amplicon size (bp)	UPL probe number	Probe recognition site
Genes of interest
GluR1	Flip	NM_001113325.1	GCAAGGACTCCGGAAGTAAGG	TCTCTCCACTGCCACTTCCT	261	51	GGCAGGAG
	Flop	NM_008165.3	GGGAGGTGACTCCAAGGACAA	TCTCTCCACTGCCACTTCCT	257
GluR2	Flip	NM_001083806.1	GCCAAGGACTCGGGAAGTA	TGGGTTAATATTCTGTGCATTCTTTG	186	67	TGCTGGAG
	Flop	NM_013540.2	GGGGAGGTGATTCCAAGG	TGGGTTAATATTCTGTGCATTCTTTG	182
GluR3	Flip	NM_016886.3	GGAATGTGGAGCCAAGGAC	TGGCAGGAGCAGGCTTAAA	206	45	CTGGGGCT
	Flop	AB102775.1	GCGGTGACTCCAAGGACAA	TGGCAGGAGCAGGCTTAAA	189
GluR4	Flip	NM_019691.4	AAGGACTCGGGAAGCAAGGA	TCTTATGGCTTCGGAAAAAGTCA	177	37	CCAGGGCA
	Flop	NM_001113180.1	GGGAGGTGACTCCAAGGACAA	TCTTATGGCTTCGGAAAAAGTCA	175
Housekeeping genes
HPRT	N/A	NM_013556.2	GGAGCGGTAGCACCTCCT	CTGGTTCATCATCGCTAATCAC	116	69	CTTCCTCC
EIF4a2	Variants 1, 2	NM_013506.2	CGATCTACCTACCAATCGTGAA	ACCTTTCCTCCCAAATCGAC	70	53	TGGCAGAG
ATP5b	N/A	NM_016774.3	GGCACAATGCAGGAAAGG	TCAGCAGGCACATAGATAGCC	77	77	CCACCACC

UPL, Universal probe library.