Figure 11.

Notch inhibition by R04929097 does not increase NPC proliferation in Rockout-treated retinas at 72 h of light treatment. A–L, Single z-plane confocal images of light-damaged albino;Tg[gfap:EGFP]^nt11 zebrafish that were intraperitoneally injected either with R04929097 (columns 3 and 4) or DMSO (columns 1 and 2) before exposing them to either Rockout (column 2 and 4) or DMSO (columns 1 and 3) at 28 h of light treatment. Retinas collected at 72 h of light treatment were immunocytochemically labeled for PCNA (A–D, I–L) and GFP (E–L) and counterstained with DAPI (I–L). M, Histogram depicting the number of PCNA-positive cells in the INL and ONL at 72 h of light treatment, revealing that treating Rockout-exposed zebrafish with the γ-secretase inhibitor R04929097 did not increase the number of proliferating cells significantly. N, Histogram revealing that the number of gfap:EGFP-positive cells that were PCNA-negative was significantly decreased in the R04929097-treated DMSO- or Rockout-exposed zebrafish compared with those that received DMSO, which indicated that more Müller glia were recruited into the cell cycle in response to R04929097. Data are shown as mean ± SE, n ≥ 13, ANOVA followed by Tukey's post hoc test, *p < 0.05 as indicated by bars spanning between the treatment groups (M, N). n.s., Not significant; GCL, ganglion cell layer. Scale bar, 20 μm for A–L.